Robust survival of isolated bovine adrenal chromaffin cells following intrastriatal transplantation: a novel hypothesis of adrenal graft viability
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Robust survival of isolated bovine adrenal chromaffin cells following intrastriatal transplantation: a novel hypothesis of adrenal graft viability.
Previous investigations have demonstrated that adrenal chromaffin cells survive poorly when grafted into the striatum of rodents, nonhuman primates, and patients with Parkinson's disease. This poor survival has been attributed to the low levels of endogenous NGF within the striatum. However, chromaffin cells isolated from the nonchromaffin constituents of the adrenal medulla (fibroblasts and en...
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Chromaffin cells isolated from bovine adrenals and maintained in culture were loaded with 45Ca, and the mechanism of 45Ca efflux from these cells was investigated. During exposure to a Ca-deficient, Mg (1 mM)-containing medium, replacement of NaCl with sucrose, choline Cl, or Tris Cl reduced the rate of 45Ca efflux by approx. 30-40%, showing the presence of a Na-dependent 45Ca efflux mechanism....
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Chromaffin cells were isolated from bovine adrenal medullae and maintained in primary culture. After prelabeling with 32PO4, exposure of the chromaffin cells to acetylcholine increased the phosphorylation of a Mr approximately equal to 100,000 protein and a Mr approximately equal to 60,000 protein (tyrosine hydroxylase), visualized after separation of total cellular proteins in naDodSO4/polyacr...
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We studied endocytosis in chromaffin cells with both perforated patch and whole cell configurations of the patch clamp technique using cell capacitance measurements in combination with amperometric catecholamine detection. We found that chromaffin cells exhibit two relatively rapid, kinetically distinct forms of stimulus-coupled endocytosis. A more prevalent "compensatory" retrieval occurs repr...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1993
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.13-10-04496.1993